Messier 64 earned its nickname because of a dark band of absorbing dust in front of its eye-like bright nucleus.
The photograph marks the 15th anniversary of the telescope's first light.
The conjunction was visible shortly after sunset, with the two objects shining low in the western sky.
The joint NASA/ESA mission searches for planets in outer space, potentially looking for a world like Earth.
It is located 45 million light-years away in the constellation Cetus, known as the Whale.
The Hubble Space Telescope captured a pair of “cosmic lightsabers” on Star Wars Day.
Hubble was launched on April 24, 1990, aboard the Space Shuttle Discovery.
The telescope revealed a new observation of a star-formation region called the Trifid Nebula.
Researchers hail the achievement as a major milestone in cosmology, with the data now available for global scientific analysis.
His fascination began when watching Neil Armstrong land on the moon in 1969 during the Apollo 11 mission.